diff --git a/tests/profiling_v2/collector/test_threading.py b/tests/profiling_v2/collector/test_threading.py
index 11bfc1acfce..57c6e274053 100644
--- a/tests/profiling_v2/collector/test_threading.py
+++ b/tests/profiling_v2/collector/test_threading.py
@@ -2,6 +2,7 @@
 import glob
 import os
 import threading
+import time
 from typing import Callable
 from typing import List
 from typing import Optional
@@ -1013,6 +1014,10 @@ def test_upload_resets_profile(self) -> None:
         with self.collector_class(capture_pct=100):
             with self.lock_class():  # !CREATE! !ACQUIRE! !RELEASE! test_upload_resets_profile
                 pass
+
+        # Wait for collector to fully stop before uploading
+        time.sleep(0.05)
+
         ddup.upload()  # pyright: ignore[reportCallIssue]
 
         linenos: LineNo = get_lock_linenos("test_upload_resets_profile", with_stmt=True)
@@ -1037,11 +1042,18 @@ def test_upload_resets_profile(self) -> None:
         )
 
         # Now we call upload() again, and we expect the profile to be empty
+        num_files_before_second_upload: int = len(glob.glob(self.output_filename + ".*"))
+
         ddup.upload()  # pyright: ignore[reportCallIssue]
-        # parse_newest_profile raises an AssertionError if the profile doesn't
-        # have any samples
-        with pytest.raises(AssertionError):
-            pprof_utils.parse_newest_profile(self.output_filename)
+
+        time.sleep(0.05)
+
+        num_files_after_second_upload: int = len(glob.glob(self.output_filename + ".*"))
+
+        # If a new file was created, it should be empty and parse_newest_profile should raise
+        if num_files_before_second_upload != num_files_after_second_upload:
+            with pytest.raises(AssertionError):
+                pprof_utils.parse_newest_profile(self.output_filename)
 
 
 class TestThreadingLockCollector(BaseThreadingLockCollectorTest):