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more fixes for CRAN release
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README.md

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Status](https://travis-ci.com/grunwaldlab/metacoder.png?branch=master)](https://travis-ci.com/grunwaldlab/metacoder?branch=master)
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[![codecov.io](https://codecov.io/github/grunwaldlab/metacoder/coverage.svg?branch=master)](https://codecov.io/github/grunwaldlab/metacoder?branch=master)
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[![Downloads from Rstudio mirror per
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month](http://cranlogs.r-pkg.org/badges/metacoder)](http://www.r-pkg.org/pkg/metacoder)
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month](http://cranlogs.r-pkg.org/badges/metacoder)](https://www.r-pkg.org:443/pkg/metacoder)
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[![Downloads from Rstudio
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mirror](http://cranlogs.r-pkg.org/badges/grand-total/metacoder)](http://www.r-pkg.org/pkg/metacoder)
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mirror](http://cranlogs.r-pkg.org/badges/grand-total/metacoder)](https://www.r-pkg.org:443/pkg/metacoder)
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[![CRAN
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version](http://www.r-pkg.org/badges/version/metacoder)](https://cran.r-project.org/package=metacoder)
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microbiome bioinformatics on data in the `taxmap` format defined by the
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`taxa` package, such as:
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- Summing read counts/abundance per taxon
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- Converting counts to proportions and rarefaction of counts using
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- Summing read counts/abundance per taxon
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- Converting counts to proportions and rarefaction of counts using
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`vegan`
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- Comparing the abundance (or other characteristics) of groups of
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- Comparing the abundance (or other characteristics) of groups of
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samples (e.g., experimental treatments) per taxon
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- Combining data for groups of samples
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- Simulated PCR, via EMBOSS primersearch, for testing primer
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- Combining data for groups of samples
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- Simulated PCR, via EMBOSS primersearch, for testing primer
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specificity and coverage of taxonomic groups
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- Converting common microbiome formats for data and reference
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- Converting common microbiome formats for data and reference
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databases into the objects defined by the `taxa` package.
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- Converting to and from the `phyloseq` format and the `taxa` format
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- Converting to and from the `phyloseq` format and the `taxa` format
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## Installation
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If you use metcoder in a publication, please cite our [article in PLOS
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Computational
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Biology](http://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1005404):
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Biology](https://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1005404):
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Foster ZSL, Sharpton TJ, Grünwald NJ (2017) Metacoder: An R package for
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visualization and manipulation of community taxonomic diversity data.
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Metacoder is under active development and many new features are planned.
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Some improvements that are being explored include:
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- Barcoding gap analysis and associated plotting functions
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- A function to aid in retrieving appropriate sequence data from NCBI
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- Barcoding gap analysis and associated plotting functions
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- A function to aid in retrieving appropriate sequence data from NCBI
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for *in silico* PCR from whole genome sequences
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- Graphing of different node shapes in heat trees, possibly including
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- Graphing of different node shapes in heat trees, possibly including
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pie graphs or [PhyloPics](http://phylopic.org/).
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- Adding the ability to plot specific edge lengths in the heat trees
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- Adding the ability to plot specific edge lengths in the heat trees
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so they can be used for phylogenetic trees.
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- Adding more data import and export functions to make parsing and
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- Adding more data import and export functions to make parsing and
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writing common formats easier.
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To see the details of what is being worked on, check out the

cran-comments.md

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0 errors | 0 warnings | 0 notes
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### Rhub: Ubuntu Linux 20.04.1 LTS, R-release, GCC
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### Rhub: Windows Server 2008 R2 SP1, R-devel, 32/64 bit
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0 errors | 0 warnings | 0 notes
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### All testing platforms using R devel (rhub, winbuilder)
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Some bioconductor packages in Suggests seem to not be available for R 4.2. I get the following error on R devel builds:
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Error: Bioconductor does not yet build and check packages for R version 4.2
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However, all of the bioconductor pacakges are optional and not needed for most functionality.
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However, all of the bioconductor packages are optional and not needed for most functionality.
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man/metacoder.Rd

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man/primersearch.Rd

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man/primersearch_raw.Rd

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vignettes/introduction.Rmd

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%\VignetteEncoding{UTF-8}
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---
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See the documentation at http://grunwaldlab.github.io/metacoder_documentation.
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See the documentation at https://grunwaldlab.github.io/metacoder_documentation/.
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